Evaluation of inapparent nosocomial severe acute respiratory syndrome coronavirus infection in Vietnam by use of highly specific recombinant truncated nucleocapsid protein-based enzyme-linked immunosorbent assay.
Identifieur interne : 004841 ( Main/Exploration ); précédent : 004840; suivant : 004842Evaluation of inapparent nosocomial severe acute respiratory syndrome coronavirus infection in Vietnam by use of highly specific recombinant truncated nucleocapsid protein-based enzyme-linked immunosorbent assay.
Auteurs : Fuxun Yu [Japon] ; Mai Quynh Le ; Shingo Inoue ; Hong Thi Cam Thai ; Futoshi Hasebe ; Maria Del Carmen Parquet ; Kouichi MoritaSource :
- Clinical and diagnostic laboratory immunology [ 1071-412X ] ; 2005.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (sang), Humains, Infection croisée (immunologie), Infection croisée (virologie), Infection croisée (épidémiologie), Protéines nucléocapside (immunologie), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (épidémiologie), Test ELISA, Vietnam, Virus du SRAS (génétique), Virus du SRAS (immunologie), Études séroépidémiologiques.
- MESH :
- génétique : Virus du SRAS.
- immunologie : Infection croisée, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS.
- sang : Anticorps antiviraux.
- virologie : Infection croisée.
- épidémiologie : Infection croisée, Syndrome respiratoire aigu sévère.
- Humains, Test ELISA, Vietnam, Études séroépidémiologiques.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Cross Infection (epidemiology), Cross Infection (immunology), Cross Infection (virology), Enzyme-Linked Immunosorbent Assay, Humans, Nucleocapsid Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), Seroepidemiologic Studies, Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (immunology), Vietnam.
- MESH :
- chemical , blood : Antibodies, Viral.
- geographic : Vietnam.
- epidemiology : Cross Infection, Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- immunology : Cross Infection, Nucleocapsid Proteins, SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Cross Infection.
- Enzyme-Linked Immunosorbent Assay, Humans, Seroepidemiologic Studies.
Abstract
Severe acute respiratory syndrome (SARS) is a recently emerged human disease associated with pneumonia. Inapparent infection with SARS coronavirus (CoV) is not well characterized. To develop a safe, simple, and reliable screening method for SARS diagnosis and epidemiological study, two recombinant SARS-CoV nucleocapsid proteins (N' protein and (N)Delta(121) protein) were expressed in Escherichia coli, purified by affinity chromatography, and used as antigens for indirect, immunoglobulin G enzyme-linked immunosorbent assays (ELISA). Serum samples collected from healthy volunteers and SARS patients in Vietnam were used to evaluate the newly developed methods. The N' protein-based ELISA showed a highly nonspecific reaction. The (N)Delta(121) protein-based ELISA, with a nonspecific reaction drastically reduced compared to that of the nearly-whole-length N' protein-based ELISA, resulted in higher rates of positive reactions, higher titers, and earlier detection than the SARS-CoV-infected cell lysate-based ELISA. These results indicate that our newly developed SARS-CoV (N)Delta(121) protein-based ELISA is not only safe but also a more specific and more sensitive method to diagnose SARS-CoV infection and hence a useful tool for large-scale epidemiological studies. To identify inapparent SARS-CoV infections, serum samples collected from health care workers (HCWs) in Vietnam were screened by the (N)Delta(121) protein-based ELISA, and positive samples were confirmed by a virus neutralization test. Four out of 149 HCWs were identified to have inapparent SARS-CoV infection in Vietnam, indicating that subclinical SARS-CoV infection in Vietnam is rare but does exist.
DOI: 10.1128/CDLI.12.7.848-854.2005
PubMed: 16002634
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002653
- to stream PubMed, to step Curation: 002653
- to stream PubMed, to step Checkpoint: 002722
- to stream Ncbi, to step Merge: 001046
- to stream Ncbi, to step Curation: 001046
- to stream Ncbi, to step Checkpoint: 001046
- to stream Main, to step Merge: 004B39
- to stream Main, to step Curation: 004841
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of inapparent nosocomial severe acute respiratory syndrome coronavirus infection in Vietnam by use of highly specific recombinant truncated nucleocapsid protein-based enzyme-linked immunosorbent assay.</title><author><name sortKey="Yu, Fuxun" sort="Yu, Fuxun" uniqKey="Yu F" first="Fuxun" last="Yu">Fuxun Yu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523</wicri:regionArea><wicri:noRegion>Nagasaki 852-8523</wicri:noRegion></affiliation></author><author><name sortKey="Le, Mai Quynh" sort="Le, Mai Quynh" uniqKey="Le M" first="Mai Quynh" last="Le">Mai Quynh Le</name></author><author><name sortKey="Inoue, Shingo" sort="Inoue, Shingo" uniqKey="Inoue S" first="Shingo" last="Inoue">Shingo Inoue</name></author><author><name sortKey="Thai, Hong Thi Cam" sort="Thai, Hong Thi Cam" uniqKey="Thai H" first="Hong Thi Cam" last="Thai">Hong Thi Cam Thai</name></author><author><name sortKey="Hasebe, Futoshi" sort="Hasebe, Futoshi" uniqKey="Hasebe F" first="Futoshi" last="Hasebe">Futoshi Hasebe</name></author><author><name sortKey="Del Carmen Parquet, Maria" sort="Del Carmen Parquet, Maria" uniqKey="Del Carmen Parquet M" first="Maria" last="Del Carmen Parquet">Maria Del Carmen Parquet</name></author><author><name sortKey="Morita, Kouichi" sort="Morita, Kouichi" uniqKey="Morita K" first="Kouichi" last="Morita">Kouichi Morita</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16002634</idno><idno type="pmid">16002634</idno><idno type="doi">10.1128/CDLI.12.7.848-854.2005</idno><idno type="wicri:Area/PubMed/Corpus">002653</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002653</idno><idno type="wicri:Area/PubMed/Curation">002653</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002653</idno><idno type="wicri:Area/PubMed/Checkpoint">002722</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002722</idno><idno type="wicri:Area/Ncbi/Merge">001046</idno><idno type="wicri:Area/Ncbi/Curation">001046</idno><idno type="wicri:Area/Ncbi/Checkpoint">001046</idno><idno type="wicri:doubleKey">1071-412X:2005:Yu F:evaluation:of:inapparent</idno><idno type="wicri:Area/Main/Merge">004B39</idno><idno type="wicri:Area/Main/Curation">004841</idno><idno type="wicri:Area/Main/Exploration">004841</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluation of inapparent nosocomial severe acute respiratory syndrome coronavirus infection in Vietnam by use of highly specific recombinant truncated nucleocapsid protein-based enzyme-linked immunosorbent assay.</title><author><name sortKey="Yu, Fuxun" sort="Yu, Fuxun" uniqKey="Yu F" first="Fuxun" last="Yu">Fuxun Yu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523</wicri:regionArea><wicri:noRegion>Nagasaki 852-8523</wicri:noRegion></affiliation></author><author><name sortKey="Le, Mai Quynh" sort="Le, Mai Quynh" uniqKey="Le M" first="Mai Quynh" last="Le">Mai Quynh Le</name></author><author><name sortKey="Inoue, Shingo" sort="Inoue, Shingo" uniqKey="Inoue S" first="Shingo" last="Inoue">Shingo Inoue</name></author><author><name sortKey="Thai, Hong Thi Cam" sort="Thai, Hong Thi Cam" uniqKey="Thai H" first="Hong Thi Cam" last="Thai">Hong Thi Cam Thai</name></author><author><name sortKey="Hasebe, Futoshi" sort="Hasebe, Futoshi" uniqKey="Hasebe F" first="Futoshi" last="Hasebe">Futoshi Hasebe</name></author><author><name sortKey="Del Carmen Parquet, Maria" sort="Del Carmen Parquet, Maria" uniqKey="Del Carmen Parquet M" first="Maria" last="Del Carmen Parquet">Maria Del Carmen Parquet</name></author><author><name sortKey="Morita, Kouichi" sort="Morita, Kouichi" uniqKey="Morita K" first="Kouichi" last="Morita">Kouichi Morita</name></author></analytic><series><title level="j">Clinical and diagnostic laboratory immunology</title><idno type="ISSN">1071-412X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Cross Infection (epidemiology)</term><term>Cross Infection (immunology)</term><term>Cross Infection (virology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Nucleocapsid Proteins (immunology)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Seroepidemiologic Studies</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Vietnam</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (sang)</term><term>Humains</term><term>Infection croisée (immunologie)</term><term>Infection croisée (virologie)</term><term>Infection croisée (épidémiologie)</term><term>Protéines nucléocapside (immunologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Test ELISA</term><term>Vietnam</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Études séroépidémiologiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Vietnam</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Cross Infection</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Infection croisée</term><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Cross Infection</term><term>Nucleocapsid Proteins</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infection croisée</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cross Infection</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Infection croisée</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Seroepidemiologic Studies</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Test ELISA</term><term>Vietnam</term><term>Études séroépidémiologiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a recently emerged human disease associated with pneumonia. Inapparent infection with SARS coronavirus (CoV) is not well characterized. To develop a safe, simple, and reliable screening method for SARS diagnosis and epidemiological study, two recombinant SARS-CoV nucleocapsid proteins (N' protein and (N)Delta(121) protein) were expressed in Escherichia coli, purified by affinity chromatography, and used as antigens for indirect, immunoglobulin G enzyme-linked immunosorbent assays (ELISA). Serum samples collected from healthy volunteers and SARS patients in Vietnam were used to evaluate the newly developed methods. The N' protein-based ELISA showed a highly nonspecific reaction. The (N)Delta(121) protein-based ELISA, with a nonspecific reaction drastically reduced compared to that of the nearly-whole-length N' protein-based ELISA, resulted in higher rates of positive reactions, higher titers, and earlier detection than the SARS-CoV-infected cell lysate-based ELISA. These results indicate that our newly developed SARS-CoV (N)Delta(121) protein-based ELISA is not only safe but also a more specific and more sensitive method to diagnose SARS-CoV infection and hence a useful tool for large-scale epidemiological studies. To identify inapparent SARS-CoV infections, serum samples collected from health care workers (HCWs) in Vietnam were screened by the (N)Delta(121) protein-based ELISA, and positive samples were confirmed by a virus neutralization test. Four out of 149 HCWs were identified to have inapparent SARS-CoV infection in Vietnam, indicating that subclinical SARS-CoV infection in Vietnam is rare but does exist.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Del Carmen Parquet, Maria" sort="Del Carmen Parquet, Maria" uniqKey="Del Carmen Parquet M" first="Maria" last="Del Carmen Parquet">Maria Del Carmen Parquet</name><name sortKey="Hasebe, Futoshi" sort="Hasebe, Futoshi" uniqKey="Hasebe F" first="Futoshi" last="Hasebe">Futoshi Hasebe</name><name sortKey="Inoue, Shingo" sort="Inoue, Shingo" uniqKey="Inoue S" first="Shingo" last="Inoue">Shingo Inoue</name><name sortKey="Le, Mai Quynh" sort="Le, Mai Quynh" uniqKey="Le M" first="Mai Quynh" last="Le">Mai Quynh Le</name><name sortKey="Morita, Kouichi" sort="Morita, Kouichi" uniqKey="Morita K" first="Kouichi" last="Morita">Kouichi Morita</name><name sortKey="Thai, Hong Thi Cam" sort="Thai, Hong Thi Cam" uniqKey="Thai H" first="Hong Thi Cam" last="Thai">Hong Thi Cam Thai</name></noCountry><country name="Japon"><noRegion><name sortKey="Yu, Fuxun" sort="Yu, Fuxun" uniqKey="Yu F" first="Fuxun" last="Yu">Fuxun Yu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004841 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004841 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:16002634
|texte= Evaluation of inapparent nosocomial severe acute respiratory syndrome coronavirus infection in Vietnam by use of highly specific recombinant truncated nucleocapsid protein-based enzyme-linked immunosorbent assay.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16002634" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |